The present study was performed to investigate the effect of bicyclol, a synthetic anti-hepatitis\ndrug with anti-oxidative and anti-inflammatory properties, on anti-tuberculosis (anti-TB) drug-induced\nliver injury and related mechanisms in rats. Bicyclol was given to rats by gavage 2 h before the oral\nadministration of an anti-TB drug once a day for 30 days. Liver injury was evaluated by biochemical\nand histopathological examinations. Lipid peroxidation, mitochondrial function, and the activity\nof antioxidants were measured by spectrophotometric methods. Cytokines expression and CYP2E1\nactivity were determined by ELISA assay and liquid chromatographyââ?¬â??tandem mass spectrometry\n(LCââ?¬â??MS/MS) analysis. The expressions of hepatic CYP2E1 and hepatocyte growth factor (HGF) were\nassessed byWestern blotting. As a result, bicyclol significantly protected against anti-TB drug-induced\nliver injury by reducing the elevated serum aminotransferases levels and accumulation of hepatic\nlipids. Meanwhile, the histopathological changes were also attenuated in rats. The protective effect of\nbicyclol on anti-TB drug-induced hepatotoxicity was mainly due to its ability to attenuate oxidative\nstress, suppress the inflammatory cytokines and CYP2E1 expression, up-regulate the expression of\nHGF, and improve mitochondrial function. Furthermore, administration of bicyclol had no significant\neffect on the plasma pharmacokinetics of the anti-TB drug in rats.
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